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id="hvjh9xd" class="ny_newsdetail_con"> <strong><a href="http://m.cn-liling.com/">微生物檢測(cè)</a></strong>的三種情況<br /> 1 ．一般性狀<br />　　<strong>微生物檢測(cè)</strong>的一般性狀包括外觀、厚度、 pH 值等。剛配制的液體培養(yǎng)基，其外觀應(yīng)透明、清亮、無(wú)混濁、無(wú)沉淀，顏色符合要求。新鮮的固體培養(yǎng)基應(yīng)具有特定的顏色，表面濕潤(rùn)但無(wú)水汽、平整、光潔無(wú)凹坑和氣泡。整塊平板厚薄均勻，一般厚度在 3mm ，但 MH 平板的厚度不得小于 4mm 。斜面的長(zhǎng)度不得超過(guò)試管長(zhǎng)度的 2 ／ 3 。應(yīng)經(jīng)常觀察貯存培養(yǎng)基的質(zhì)量是否合格。若試管內(nèi)液體培養(yǎng)基液面降低，表明水分蒸發(fā)，培養(yǎng)基濃縮。當(dāng)固體培養(yǎng)基發(fā)生顏色改變，表面干燥，出現(xiàn)裂紋或邊緣的瓊脂與平板或試管分離時(shí)，應(yīng)不再使用。培養(yǎng)基的 pH 是細(xì)菌生長(zhǎng)的重要條件之一，一般而言合格培養(yǎng)基的 pH 應(yīng)在規(guī)定值上下 0.2 的范圍內(nèi)。<br /> 2 ．無(wú)菌試驗(yàn)<br />　　新制備的<strong>微生物檢測(cè)</strong>要按批號(hào)隨機(jī)抽取一定數(shù)量的樣品作無(wú)菌試驗(yàn)。對(duì)于壓力蒸氣滅菌后傾注的固體培養(yǎng)基，抽樣后放 35 ℃ ± 1 ℃ 溫箱培養(yǎng) 24-48h ；滅菌后經(jīng)無(wú)菌操作分裝的液體培養(yǎng)基要全部放入 35 ℃ ± 1 ℃ 溫箱內(nèi)培養(yǎng) 24h ；對(duì)有些無(wú)需高壓滅菌只需煮沸消毒的選擇性培養(yǎng)基要取部分瓊脂，放入無(wú)菌肉湯管培養(yǎng) 24h ：上述試驗(yàn)證實(shí)無(wú)細(xì)菌生長(zhǎng)時(shí)才算合格。若有細(xì)菌生長(zhǎng)，說(shuō)明培養(yǎng)基制備過(guò)程中已受雜菌污染，除了尋找原因外，應(yīng)不再使用。<br /> 3 ．細(xì)菌生長(zhǎng)試驗(yàn)<br />　　所有的<strong>微生物檢測(cè)</strong>在使用前除了做無(wú)菌試驗(yàn)外還必須做細(xì)菌生長(zhǎng)試驗(yàn)以測(cè)定培養(yǎng)基性能是否符合要求。要用已知的標(biāo)準(zhǔn)菌株按照 NCCLS 推薦的方法作質(zhì)控。質(zhì)控所需的標(biāo)準(zhǔn)菌株分 2 種：一種是已知的可在某種培養(yǎng)基上生長(zhǎng)并產(chǎn)生典型生物學(xué)性狀的，對(duì)培養(yǎng)基中的某種物質(zhì)產(chǎn)生陽(yáng)性反應(yīng)的菌株；另一種是用已知的不能在某種培養(yǎng)基上生長(zhǎng)或?qū)ε囵B(yǎng)基中的某種物質(zhì)產(chǎn)生陰性生化反應(yīng)的菌株。表 2 列出 10 種常用固體培養(yǎng)基的質(zhì)量鑒定標(biāo)準(zhǔn)及所需標(biāo)準(zhǔn)菌株。<br />　　無(wú)論是廠家的成品<strong>微生物檢測(cè)</strong>還是實(shí)驗(yàn)室自制的培養(yǎng)基，都應(yīng)經(jīng)過(guò)上述三方面的檢驗(yàn)才能證實(shí)其質(zhì)量是否合格。廠家應(yīng)將所做的試驗(yàn)內(nèi)容形成書(shū)面的質(zhì)量鑒定送交客戶保存。實(shí)驗(yàn)室自行配制過(guò)程中應(yīng)對(duì)上述鑒定內(nèi)容有明確記載。此外還應(yīng)對(duì)培養(yǎng)基配制原料的性狀、批號(hào)、失效期以及配制過(guò)程各個(gè)環(huán)節(jié)的操作形成記錄。 </div> <div id="hvjh9xd" class="ny_PrevNext"> <div id="hvjh9xd" class="Prev">上一篇： <a 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