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autocomplete="off" onfocus="if(this.value=='請輸入您要查詢的產(chǎn)品'){this.value='';this.style.color='#a4ada9' }" onblur="if(this.value=='') if(this.value=='')" style="color: rgb(164, 173, 169);"> <input class="submit fl" type="submit" name="Submit" value=""> </form> </div> </div> </div>   <div id="hvjh9xd" class="ny_content"> <div id="hvjh9xd" class="w1200 clear"> <div id="hvjh9xd" class="ny_bread_nav"><img src="/skins/290838/images/position.png" />當(dāng)前位置：<a href="/">首頁</a> > <a href="/article.html">技術(shù)文章</a> > 微生物檢測的檢驗(yàn)方法小結(jié)</div> </div> <div id="hvjh9xd" class="ny_content_bd"> <div id="hvjh9xd" class="w1200 clear"> <div id="hvjh9xd" class="ny_newsdetail clear"> <h1 class="ny_newsdetail_title">微生物檢測的檢驗(yàn)方法小結(jié)</h1> <div id="hvjh9xd" class="ny_newsdetail_tips">更新時(shí)間：2014-10-13 | 點(diǎn)擊率：2435</div> <div id="hvjh9xd" class="ny_newsdetail_con"> <strong><a href="http://m.cn-liling.com/">微生物檢測</a></strong>的檢驗(yàn)方法<div> <strong> 微生物檢測</strong>方法一：沉降法</div><div> 將盛有培養(yǎng)基的平皿放在空氣中暴露一定時(shí)間，經(jīng)培養(yǎng)后計(jì)算出其上所生長的菌落數(shù)。此法簡單，使用普遍，由于只有一定大小的顆粒在一定時(shí)間內(nèi)才能降到培養(yǎng)基上，因此，所測得微生物數(shù)量欠準(zhǔn)確，檢驗(yàn)結(jié)果比實(shí)際存在數(shù)量少，并且也無法測定空氣質(zhì)量，所以，僅能粗略計(jì)算空氣污染及了解被測趣味生物的種類。</div><div> 【檢驗(yàn)方法】</div><div> （1）將一直被好的營養(yǎng)瓊脂、查氏培養(yǎng)基及高氏1號三種平板置于待測地點(diǎn)，打開皿蓋在空氣中暴露5~10min。</div><div> （2）蓋上培養(yǎng)皿蓋，置于℃培養(yǎng)24~45h，根據(jù)平板上所生長的菌落數(shù)計(jì)算出1m?或1L空氣中的細(xì)菌總數(shù)。</div><div> （3）計(jì)算，奧梅梁斯基認(rèn)為：在面積為100cm2平板瓊脂培養(yǎng)基表面上，5min降落的細(xì)菌數(shù)經(jīng)37℃培養(yǎng)24h后生長的菌落數(shù)和10L空氣中所含的細(xì)菌數(shù)相當(dāng)。根據(jù)奧氏公式計(jì)算</div><div> 式中X—每1m?空氣中的細(xì)菌個(gè)數(shù)；</div><div> 式中N—5min降落在平板上，經(jīng)37℃培養(yǎng)24h后生長的菌落數(shù)；</div><div> 式中r—平皿底半徑。</div><div> 經(jīng)測定發(fā)現(xiàn)，用奧氏公式計(jì)算的浮游細(xì)菌數(shù)比實(shí)測的浮游細(xì)菌少。原因是此公式?jīng)]有考慮塵埃粒子大小、數(shù)量、氣流情況、人員密度和活動(dòng)情況。</div><div> <strong>微生物檢測</strong>方法二：撞擊法</div><div> 以縫隙采樣器為例，用吸風(fēng)機(jī)或真空泵江含菌空氣以一定流速穿過狹縫而被抽吸到營養(yǎng)瓊脂培養(yǎng)及平板上。狹縫長度為平皿的半徑，平板與縫的間隙有2㎜，平板以一定的轉(zhuǎn)速旋轉(zhuǎn)。通常平板轉(zhuǎn)動(dòng)一周，取出置于37℃恒溫箱中培養(yǎng)的48h，根據(jù)空氣中微生物的密度可調(diào)節(jié)平板狀動(dòng)的速度。采集含菌高的空氣樣品時(shí)，平板轉(zhuǎn)動(dòng)的速度要比含菌zui低的空氣樣品的轉(zhuǎn)速快。根據(jù)取樣時(shí)間和空氣流量算出單位空氣中的含菌量。采樣器的規(guī)格各國不一，可按說明書操作。</div><div> <strong> 微生物檢測</strong>方法三：過濾法</div><div> 該法是抽取定量空氣通過一種液體吸收劑，然后取此液體定量培養(yǎng)計(jì)數(shù)出菌落數(shù)。</div><div> （1）將無菌的液體培養(yǎng)基或無菌水與真空泵相連，以每分鐘10L速度取空氣樣并劇烈震蕩，使阻流在液體中的氣溶膠或微生物均勻分散。</div><div> （2）吸取上述含菌液體吸收液1mL與融化冰冷卻到45℃左右的營養(yǎng)液瓊脂做傾注培養(yǎng)，同時(shí)做三個(gè)平行試驗(yàn)，置37℃恒溫箱中培養(yǎng)48h，計(jì)算平均菌落數(shù)。根據(jù)下列公式計(jì)算</div><div> 式中X—每1m?空氣中的細(xì)菌數(shù)；</div><div> 式中Vs—吸收液體量，mL；</div><div> 式中Va—空氣過濾量，L；</div><div> 式中N—每1mL液體培養(yǎng)基中的細(xì)菌數(shù)。</div><div> <strong>微生物檢測</strong>方法四：簡易定量測定法</div><div> 用無菌注射器定量抽取空氣，將所取空氣壓入培養(yǎng)基內(nèi)部，經(jīng)培養(yǎng)后，即可定量、定性測定空氣中微生物，此法簡單易行。</div><div> （1）將無菌固體培養(yǎng)基融化后，在50℃水浴中保溫備用。</div><div> （2）利用50~100mL無菌注射器抽取待測環(huán)境空氣20~100mL。</div><div> （3）在無菌操作下，取已融化培養(yǎng)基倒入無菌平皿中，平皿稍作傾斜，將注射器插入培養(yǎng)基深處，緩慢將空氣壓入培養(yǎng)基內(nèi)，輕輕搖勻以消除氣泡。待培養(yǎng)基凝固，置于30℃恒溫箱中培養(yǎng)3d后統(tǒng)計(jì)菌落數(shù)量，推算1L空氣所含菌量。通過菌落形態(tài)定性微生物，如統(tǒng)計(jì)霉菌數(shù)量時(shí)培養(yǎng)的時(shí)間稍許延長。應(yīng)用此法需多做平行試驗(yàn)，求其平均值以提高準(zhǔn)確性。</div> </div> <div id="hvjh9xd" class="ny_PrevNext"> <div id="hvjh9xd" class="Prev">上一篇： <a href="/Article-703643.html">安裝便攜式污泥濃度計(jì)時(shí)的注意事項(xiàng) </a></div> <div id="hvjh9xd" class="Next">下一篇： <a href="/Article-699698.html">哈希LDO傳感器帽</a></div> </div> </div> </div> </div> </div>   <div id="hvjh9xd" class="pc_foot"> <div id="hvjh9xd" class="pc_ft_main w1200 clear"> <div id="hvjh9xd" class="ft_list"> <ul> <li><b>關(guān)于我們</b><a href="/aboutus.html">公司簡介</a><a href="/news.html">新聞動(dòng)態(tài)</a><a href="/article.html">技術(shù)文章</a></li> <li><b>產(chǎn)品展示</b> <a href="/Products-18038017.html">默克德國MERCK默克符合藥典要求試劑和輔料</a> <a 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